PAI-1 RQ Kit
Detection of PAI-1 4G/5G polymorphism
PAI-1 (Plasminogen Activator Inhibitor 1) is a 52 kDa glycoprotein. It is the main inhibitor of activators of plasminogen, and hence fibrinolysis and blood clot degradation. PAI-1 gene is located on chromosome 7 and consists of 9 exons. It includes many variations. The most significant variation, however, lies in the promoter region at -675, which is a single guanine base insertion/deletion known as 4G/5G polymorphism. While 5G allele binds both transcription activator and repressor, 4G allele only binds activator and leads to higher expression of PAI-1. 4G allele is considered a predisposing factor and has been related to various clinically important conditions including deep vein thrombosis, atherosclerosis and myocardial infarction, cancer, sepsis, miscarriage and asthma.
PAI-1 RQ kit provides a ready-to-use Real-Time PCR system for detection of PAI-1 4G/5G polymorphism with StepOne machine.
Ready to Use
The PAI-1 Mix contains all necessary reagents for Real-Time PCR and is ready to use; no further reagent or mixing is required. Reactions are prepared simply by addition of the mix directly to the tubes followed by the sample DNA. Results can be viewed in Green/FAM channel melt curve.
This kit requires no internal control as any sample should be positive for either or both of mutant and wild type allels. Therefore, the main reaction ensures quality of patient sample and DNA extraction and also prevents false negative results by PCR inhibition or setup errors.
mix & controls of PAI-1 RQ Kit are manufactured in batches, and each series is distributed only after passing Quality Control tests for Specificity, Sensitivity, and Accuracy.
Easy & Quick Setup
The kit contains a template file for quick and easy setup of the Real-Time PCR instrument and no need to go through time taking manual setting, also reducing setup errors.Kit Contents Kit contains PAI-1 Mix, 5G/5G Positive control, 4G/5G Positive control, 4G/4G Negative control, PCR grade water, manual and a CD with template file. Packaging Kit is available as 24, 48 and 96 reactions of 25µl volume. Technical Support Technical support team is always available via phone or email. The team may also visit your lab for training or troubleshooting.