KRAS RQ Kit
Quantitative Detection of KRAS Transcripts
Colorectal cancer (CRC) is among the most prevalent cancers worldwide. Treatment with monoclonal antibodies against Epidermal Growth Factor Receptor (Anti-EGFR) has shown to be effective for CRC patients. However, Anti-EGFR would be in-effective in presence of some KRAS mutations including mutations in codons 12 and 13. This is the same for treating non small cell lung cancer (NSCLC) with Anti-EGFR. Therefore, determining the KRAS mutation status is essential for these patients. It should be noted that, 95% and 88% of KRAS mutations in CRC and NSCLC patients respectively are reported in codons 12 and 13 including G12A, G12C, G12D, G12R, G12S, G12V and G13D.
KRAS RQ Kit provides ready to use mixes for detection of these 7 mutations based on Real-Time PCR technology.
Advantages of KRAS RQ Kit
Ready to Use
The KRAS Mixes contain all necessary reagents for Real-Time PCR and are ready to use; no further reagent or mixing is required. Reactions are prepared simply by addition of the mix directly to the tubes followed by the sample DNA. Results can be viewed in Green/FAM channel for KRAS and Yellow/VIC channel for Controls.
examining a sample for KRAS mutations, quality of DNA should be assessed. If the results are within the desired range, then the second test for detecting KRAS mutations will be performed.
Kit also provides PCR mix and standards for assessment of controls expression as the control gene. This would evaluate quality of the patient sample, extraction DNA synthesis as well as preventing false negative results by PCR inhibition or setup errors.
mixes of KRAS RQ Kit are manufactured in batches, and each series is distributed only after passing Quality Control tests for Specificity, Sensitivity, Accuracy and Precision.
Easy & Quick Setup
Kit contains a template file for quick and easy setup of the Real-Time PCR instrument and no need to go through time taking manual setting, also reducing setup errors.
Kit contains 7PCR Mix (G12A, G12C, G12R, G12S G12V, G13D, G12D ) and one positive control and PCR grade water, manual and a CD with template file.
Kit is available as 24, 48 and 96 reactions of 25µl volume.
Technical support team is always available via phone or email. The team may also visit your lab for training or troubleshooting.
KRAS RQ Quick Guide
KRAS RQ Manual
KRAS RQ RotorGene Templates