Novin Gene

Real-Time PCR Kits

JC RQ Kit

Quantitative Detection of JC virus DNA

JC virus is a member of polyomaviruses genus with a double stranded DNA genome. BK virus belongs to this genus as well. Early infection occurs in early childhood and without clinical symptoms. Then JC virus establishes a lifelong infection usaully in kidney, central nervous system or lymphocytes. Usually about 80% of adult population are infected with this virus without noticing it. However, JCV may lead to significant consequences in patients with immune deficiency or very weak immune system such as people with HIV/AIDs, lymphoma, multiple sclerosis (MS) and transplant patients. There, JCV reactivation may result on a serious brain infection called progressive multifocal leukoencephalopathy (PML). Therefore, it is crucial to monitor JCV titre in above mentioned target group.

JC RQ kit provides a ready-to-use Real-Time PCR system for detection and quantitation of JC virus DNA with Rotor-Gene or StepOne machines.

Description

Advantages of JC RQ Kit
 

Ready to Use

The JC Mix contains all necessary reagents for Real-Time PCR and is ready to use; no further preparation or mixing is required. Reactions are prepared simply by addition of the mix directly to the tubes followed by the sample DNA. Results can be viewed in FAM/Green channel.

 Internal Control

To monitor both DNA extraction and PCR reaction, internal control should be added to the mixture of lysis buffer and patient sample. Required volume of internal control is 10% of elution buffer.

Internal Control prevents false negative results by PCR inhibition or setup errors.

Quality Control

Standards & mixes of JC RQ Kit are manufactured in batches, and each series is distributed only after passing Quality Control tests for Specificity, Sensitivity, Accuracy and Precision.

 Easy & Quick Setup

Kit contains a template file for quick and easy setup of the Real-Time PCR instrument and no need to go through time taking manual setting, also reducing setup errors.

Kit Contents
Kit contains PCR Mix and 4 quantitation
standards and internal control for JC Mix and water, manual and a CD with template file.
Packaging
Kit is available as 24, 48 and 96 reactions of 25µl volume.

Technical Support
Technical support team is always available via phone or email. The team may also visit your lab for training or troubleshooting.

JC RQ Manual
JC RQ Quick Guide
JC RQ RotorGene Templates
JC RQ StepOne Templates

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