JC RQ Kit
Quantitative Detection of JC virus DNA
JC virus is a member of polyomaviruses genus with a double stranded DNA genome. BK virus belongs to this genus as well. Early infection occurs in early childhood and without clinical symptoms. Then JC virus establishes a lifelong infection usaully in kidney, central nervous system or lymphocytes. Usually about 80% of adult population are infected with this virus without noticing it. However, JCV may lead to significant consequences in patients with immune deficiency or very weak immune system such as people with HIV/AIDs, lymphoma, multiple sclerosis (MS) and transplant patients. There, JCV reactivation may result on a serious brain infection called progressive multifocal leukoencephalopathy (PML). Therefore, it is crucial to monitor JCV titre in above mentioned target group.
JC RQ kit provides a ready-to-use Real-Time PCR system for detection and quantitation of JC virus DNA with Rotor-Gene or StepOne machines.
Ready to Use
The JC Mix contains all necessary reagents for Real-Time PCR and is ready to use; no further preparation or mixing is required. Reactions are prepared simply by addition of the mix directly to the tubes followed by the sample DNA. Results can be viewed in FAM/Green channel.
To monitor both DNA extraction and PCR reaction, internal control should be added to the mixture of lysis buffer and patient sample. Required volume of internal control is 10% of elution buffer.
Internal Control prevents false negative results by PCR inhibition or setup errors.
Standards & mixes of JC RQ Kit are manufactured in batches, and each series is distributed only after passing Quality Control tests for Specificity, Sensitivity, Accuracy and Precision.
Easy & Quick Setup
Kit contains a template file for quick and easy setup of the Real-Time PCR instrument and no need to go through time taking manual setting, also reducing setup errors.Kit Contents Kit contains PCR Mix and 4 quantitation standards and internal control for JC Mix and water, manual and a CD with template file. Packaging Kit is available as 24, 48 and 96 reactions of 25µl volume. Technical Support Technical support team is always available via phone or email. The team may also visit your lab for training or troubleshooting.