JAK2 RQ Kit
Detection of JAK2 Mutation (V617F)
JAK2 (Janus Kinase 2) is a Tyrosine Kinase located in cytoplasm with essential role in signaling pathways for cytokines and growth factors. The acquired mutation G1849T replaces valine with phenylalanine (V617F). This substitution results in constitutively active JAK2 which leads to uncontrolled cell proliferation in the absence of growth factors. This mutation is found in the majority of BCR-ABL-negative myeloproliferative disorders (MPDs) and has become a main diagnostic test for polycythemia vera (PV), essential thrombocythemia (ET) and primary myelofibrosis (PMF).
JAK2 RQ kit provides a ready-to-use Real-Time PCR system for detection of JAK2 V617F mutation with Rotor-Gene or StepOne machines.
Ready to Use
The JAK2 Mix contains all necessary reagents for Real-Time PCR and is ready to use; no further reagent or mixing is required. Reactions are prepared simply by addition of the mix directly to the tubes followed by the sample DNA. Results can be viewed in Green/FAM channel.
PCR mix detects also a housekeeping gene in Yellow/VIC channel. Internal Control ensures quality of patient sample and DNA extraction and also prevents false negative results by PCR inhibition or setup errors.
JAK2 controls & mix are manufactured in batches, and each series is distributed only after passing Quality Control tests for Specificity, Sensitivity and Accuracy.
Easy & Quick Setup
kit contains a template file for quick and easy setup of the Real-Time PCR instrument and no need to go through time taking manual setting, also reducing setup errors.
Kit contains JAK2 Mix, Positive control, Negative control, PCR grade water, manual and a CD with template file.
Kit is available as 24, 48 and 96 reactions of 25µl volume.
Technical support team is always available via phone or email. The team may also visit your lab for training or troubleshooting.