Factor XIII RQ Kit
Detection of Factor XIII (Val34Leu) Polymorphism
Factor XIII, first called fibrin stabilizing factor, was discovered in 1948. It is involved in the last stage of coagulation and participates in clot preservation. It also participates in wound repair and healing, maintaining pregnancy and acts as proangiogenesis. Among the few polymorphisms known for this factor, the Val34Leu has been studied more extensively as increases the activity of this factor and leads to change in fibrin clots. Studies have shown that this polymorphism may reduce the risk of myocardial infarction, brain infarction and venous thromboembolism (VTE). However, it also increases the risk of intracerebral hemorrhage and pulmonary embolism.
Factor XIII RQ kit provides a ready-to-use Real-Time PCR system for detection of Val34Leu polymorphism of Factor XIII with RotorGene 6000/Q or StepOne/StepOnePlus instrument.
Ready to Use
The F5 Mix contains all necessary reagents for Real-Time PCR and is ready to use; no further reagent or mixing is required. Reactions are prepared simply by addition of the mix directly to the tubes followed by the sample DNA. Results can be viewed in Scatter Graph by Allelic Discrimination Analysis.
Either of sample alleles may function for internal control as any sample should be positive for one or both of mutant and wild type allels. Therefore, the main reaction ensures quality of patient sample and DNA extraction and also prevents false negative results by PCR inhibition or setup errors.
mix & controls of Factor V RQ Kit are manufactured in batches, and each series is distributed only after passing Quality Control tests for Specificity, Sensitivity, and Accuracy.
Easy & Quick Setup
kit contains a template file for quick and easy setup of the Real-Time PCR instrument and no need to go through time taking manual setting, also reducing setup errors.Kit Contents Kit contains F13 Mix, MM Positive control, WM Positive control, WW Negative control, PCR grade water, manual and a CD with template file. Packaging Kit is available as 24, 48 and 96 reactions of 25µl volume. Technical Support Technical support team is always available via phone or email. The team may also visit your lab for training or troubleshooting.