Novin Gene

Real-Time PCR Kits

Factor V Leiden RG Kit

For Real-Time PCR Detection of Factor V Leiden (G1691A) Mutation

Factor V Leiden is an inherited disorder of blood clotting and refers to specific G1691A substitution in Factor V gene (R506Q amino acid replacement), first reported in 1994.

۳ to 8% of Caucasian population carries one copy of this mutation (heterozygous) and about 1 in 5000 carry two copies of it (homozygous). Heterozygosity for factor V Leiden is associated 4 to 8 fold increased risk of venous thromboembolism (VTE) including deep venous thrombosis (DVT) and pulmonary emboly. Factor V Leiden is also associated with a two- to threefold increase in relative risk of pregnancy loss, and possibly other pregnancy complications such as preeclampsia, fetal growth retardation and placental abruption

The diagnosis of factor V Leiden requires either the APC resistance assay as a coagulation screening test or DNA analysis of F5, the gene encoding factor V.

Factor V Leiden RG kit provides a ready-to-use Real-Time PCR system for detection of G1691A mutation with Rotor-Gene.

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Description

Advantages of F5RG Kit    

Ready to Use

The F5 Mix contains all necessary reagents for Real-Time PCR and is ready to use; no further reagent or mixing is required. Reactions are prepared simply by addition of the mix directly to the tubes followed by the sample DNA. Results can be viewed in Green/FAM channel melt curve.

 Internal Control

This kit requires no internal control as any sample should be positive for either or both of mutant and wild type allels. Therefore, the main reaction ensures quality of patient sample and DNA extraction and also prevents false negative results by PCR inhibition or setup errors.

Quality Control

mix & controls of Factor V RQ Kit are manufactured in batches, and each series is distributed only after passing Quality Control tests for Specificity, Sensitivity, and Accuracy.

 Easy & Quick Setup

The kit contains a template file for quick and easy setup of the Real-Time PCR instrument and no need to go through time taking manual setting, also reducing setup errors.

Kit Contents Kit contains F5 Mix, MM Positive control, WM Positive control, WW Negative control, PCR grade water, manual and a CD with template file. Packaging Kit is available as 24, 48 and 96 reactions of 25µl volume. Technical Support Technical support team is always available via phone or email. The team may also visit your lab for training or troubleshooting.

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