ETV6-RUNX1 RQ Kit
Quantitative Detection of ETV6-RUNX1 (TEL-AML1) Transcripts
ETV6-RUNX1 (TEL-AML1) is an abnormality resulted from 21;12 translocation. RUNX1 gene, encodes a protein involved in transcriptional control of hematopoiesis. However, as a result of this transloaction, it is repressed by ETV6-RUNX1 fusion protein.
This alteration occurs in approximately 25% of childhood ALL diagnosed between the ages of 2-10 years.
ETV6-RUNX1 (TEL-AML1) RQ provides a ready-to-use system for detection and quantitation of ETV6-RUNX1 transcripts as well as calculation of ETV6-RUNX1 percentage.
ETV6-RUNX1 RQ kit provides a ready-to-use Real-Time PCR system for detection and quantitation of ETV6-RUNX1 with Rotor-Gene or StepOne machines.
Ready to Use
The ETV6-RUNX1 and ABL Mixes contain all necessary reagents for Real-Time PCR and are ready to use; no further reagent or mixing is required. Reactions are prepared simply by addition of the mix directly to the tubes followed by the sample cDNA. Results can be viewed in Green/FAM channel for ETV6-RUNX1 and Yellow/VIC channel for ABL.
Kit also provides PCR mix and standards for assessment of ABL expression as the control gene. This would evaluate quality of the patient sample, RNA extraction and cDNA synthesis as well as preventing false negative results by PCR inhibition or setup errors.
Standards & mixes of ETV6-RUNX1 RQ Kit are manufactured in batches, and each series is distributed only after passing Quality Control tests for Specificity, Sensitivity, Accuracy and Precision.
Easy & Quick Setup
Kit contains a template file for quick and easy setup of the Real-Time PCR instrument and no need to go through time taking manual setting, also reducing setup errors.Kit Contents Kit contains PCR Mix and 5 quantitation standards for ETV6-RUNX1, PCR Mix and 4 quantitation standards for ABL, Primer and PCR grade water, manual and a CD with template file. Packaging Kit is available as 24, 48 and 96 reactions of 25µl volume. Technical Support Technical support team is always available via phone or email. The team may also visit your lab for training or troubleshooting.