Novin Gene

Real-Time PCR Kits

EBV RQ Kit

Quantitative Detection of Epstein-Barr virus (EBV) DNA

Human Herpesvirus 4 (HHV-4) known as Epstein-Barr virus (EBV) is a double-stranded DNA virus and a member of Herpesviridae family. Infection occurs mostly in early childhood and without clinical symptoms. However, infection in teens and young aldults may cause infectious mononulceosis. The primary infection is followed by the life long latent infection and about 90% of adult population are infected with this virus. EBV has also been linked to a variety of cancers including lymphoma and nasopharyngeal carcinoma. It also can be reactivated in immunocompromised hosts, after transplantation or following therapy in cancer patients leading to lymphoproliferative disorders. Therefore, viral load monitoring is important in these patients.

EBV RQ kit provides a ready-to-use Real-Time PCR system for detection and quantitation of Epstein barr virus with Rotor-Gene or StepOne machines.

Description

Advantages of EBV RQ Kit

Ready to Use

The EBV Mix contains all necessary reagents for Real-Time PCR and is ready to use; no further preparation or mixing is required. Reactions are prepared simply by addition of the mix directly to the tubes followed by the sample DNA. Results can be viewed in FAM/Green channel.

 Internal Control

PCR mix contains also specific DNA that is detected by a second probe in VIC/Yellow channel. Internal Control prevents false negative results by PCR inhibition or setup errors.

 Quality Control

Standards & mixes of EBV RQ Kit are manufactured in batches, and each series is distributed only after passing Quality Control tests for Specificity, Sensitivity, Accuracy and Precision.

 Easy & Quick Setup

Kit contains a template file for quick and easy setup of the Real-Time PCR instrument and no need to go through time taking manual setting, also reducing setup errors.

Kit Contents
Kit contains PCR Mix and 4 quantitation
standards  and internal control for EBV Mix and
water, manual and a CD with template file.
Packaging
Kit is available as 24, 48 and 96 reactions of 25µl volume.

Technical Support
Technical support team is always available via phone or email. The team may also visit your lab for training or troubleshooting.

 

EBV RQ Manual
EBV RQ Quick Guide
EBV RQ RotorGene template
EBV RQ StepOne template

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