Novin Gene

Real-Time PCR Kits

CMV RQ Kit

Quantitative Detection of Cytomegalovirus (CMV) DNA

Human Herpesvirus 5 (HHV-5) known as Cytomegalovirus (CMV) is a double-stranded DNA virus and a member of Herpesviridae family. It is a widespread pathogen with high seropositivity (about 80%) in adult population. Primary infection is usually subclinical and mostly happens in early childhood. The primary infection is followed by the latent infection and CMV persists in the infected person through life. CMV can be reactivated in immunocompromised hosts for example after stem cell, bone marrow or solid organ transplantation or in the course of HIV infection. In these patients, viral load assays are the cornerstone for the diagnosis and monitoring of CMV disease. Viral load quantification in blood allows assessing patients at risk of developing relapsing infection, early detection of active CMV infection, guiding preemptive therapy, monitoring response to therapy, determining duration of therapy and prediction of treatment failure due to emergence of resistant strains.

CMV RQ kit provides a ready-to-use Real-Time PCR system for detection and quantitation of cytomegalovirus with Rotor-Gene or StepOne machines.

Description

Advantages of CMV RQ Kit

 

Ready to Use

The CMV Mix contains all necessary reagents for Real-Time PCR and is ready to use; no further preparation or mixing is required. Reactions are prepared simply by addition of the mix directly to the tubes followed by the sample DNA. Results can be viewed in FAM/Green channel.

 Internal Control

PCR mix contains also specific DNA that is detected by a second probe in VIC/Yellow channel. Internal Control prevents false negative results by PCR inhibition or setup errors.

 Quality Control

CMV standards & mix are manufactured in batches, and each series is distributed only after passing Quality Control tests for Specificity, Sensitivity, Accuracy and Precision.

 Easy & Quick Setup

The kit contains a template file for quick and easy setup of the Real-Time PCR instrument and no need to go through time consuming process of manual setting, also reducing setup errors.

Kit Contents

Kit contains CMV Mix, 4 quantitation standards, PCR grade water, manual and a CD with template file.

Packaging

Kit is available as 24, 48 and 96 reactions of 25µl volume.

Technical Support

Technical support team is always available via phone or email. The team may also visit your lab for training or troubleshooting.

CMV RQ Quick Guide
CMV RQ Manual
CMV RQ StepOne Templates
CMV RQ RotorGene Templates

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