BK RQ Kit
Quantitative Detection of BK
BK virus is a member of polyomaviruses genus with a double stranded DNA genome. The virus is widespread with a seroprevalence of up to 90% in adults. Infection usually happens during the childhood and is either asymptomatic or with mild symptoms. Following initial infection, virus persists as a lifelong latent infection. BKV can cause significant consequences in renal or bone marrow transplant patients including polyomavirus associated nephropathy (PVAN) and also graft loss. Quantitative monitoring of BKV load in plasma or urine of transplant recipients is essential and a load of 10,000 copies/ml of plasma or 10 million copies/ml of urine for 3 weeks or more is an early indication of PVAN.
BK RQ kit provides a ready-to-use Real-Time PCR system for detection and quantitation of BK virus with Rotor-Gene or StepOne machines.
Ready to Use
The BK Mix contains all necessary reagents for Real-Time PCR and is ready to use; no further reagent or mixing is required. Reactions are prepared simply by addition of the mix directly to the tubes followed by the sample DNA. Results can be viewed in Green/FAM channel.
PCR mix contains also specific DNA that is detected by a second probe in Yellow/VIC channel. Internal Control prevents false negative results by PCR inhibition or setup errors.
Standards & mixes of BK RQ Kit are manufactured in batches, and each series is distributed only after passing Quality Control tests for Specificity, Sensitivity, Accuracy and Precision.
Easy & Quick Setup
Kit contains a template file for quick and easy setup of the Real-Time PCR instrument and no need to go through time taking manual setting, also reducing setup errors.Kit Contents Kit contains PCR Mix and 4 quantitation standards and for BK Mix and water, manual and a CD with template file. Packaging Kit is available as 24, 48 and 96 reactions of 25µl volume. Technical Support Technical support team is always available via phone or email. The team may also visit your lab for training or troubleshooting.
BK RQ Manual
BK RQ Quick Guide
BK RQ RotorGene Templates
BK RQ StepOne Templates