AML1-ETO RQ Kit
Quantitative Detection of AML1-ETO Transcripts
AML1-ETO is the most common chromosomal aberration in de novo acute myeloid leukemia (AML) patients. This abnormality is resulted from t(8;21)(q22;q22) translocation. The translocation, produces a fusion protein which inhibits myeloid transcriptional factors and as a result, cellular differentiation is blocked. This alteration occurs in approximately 7% of adult and 12% of pediatric with AML.
AML1-ETO RQ provides a ready-to-use system for detection and quantitation of AML1-ETO transcripts as well as calculation of AML1-ETO percentage.This kit is based on Real-Time PCR technology.
Ready to Use
The AML1-ETO and ABL Mixes contain all necessary reagents for Real-Time PCR and are ready to use; no further reagent or mixing is required. Reactions are prepared simply by addition of the mix directly to the tubes followed by the sample cDNA. Results can be viewed in Green/FAM channel for AML1-ETO and Yellow/VIC channel for ABL.
Kit also provides PCR mix and standards for assessment of ABL expression as the control gene. This would evaluate quality of the patient sample, RNA extraction and cDNA synthesis as well as preventing false negative results by PCR inhibition or setup errors.
Standards & mixes of AML1-ETO RQ Kit are manufactured in batches, and each series is distributed only after passing Quality Control tests for Specificity, Sensitivity, Accuracy and Precision.
Easy & Quick Setup
Kit contains a template file for quick and easy setup of the Real-Time PCR instrument and no need to go through time taking manual setting, also reducing setup errors.Kit Contents Kit contains PCR Mix and 5 quantitation standards for AML1-ETO, PCR Mix and 4 quantitation standards for ABL, Primer and PCR grade water, manual and a CD with template file. Packaging Kit is available as 24, 48 and 96 reactions of 25µl volume. Technical Support Technical support team is always available via phone or email. The team may also visit your lab for training or troubleshooting.
AML1-ETO RQ Quick Guide
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