TB RQ Kit
Quantitative Detection of M. Tuberculosis DNA
Tuberculosis (TB) is one of the major public health problems worldwide and results in about 2 million deaths annually. This infection is caused by a group of closely related species and subspecies of intracellular pathogens called Mycobacterium tuberculosis complex (MTC) including M. tuberculosis, M. bovis and M. bovis BCG.
While bacterial culturing is still the gold standard for TB diagnosis, Real-Time PCR is increasingly used as an alternative due to its rapid, sensitive and specific results. It also provides the highest sensitivity and widest dynamic range among other methods. In Real-Time PCR, application of fluorescent dye labeled probes allows detection of amplified product. Analysis of fluorescent kinetics also leads to quantification of the target sequence in the reaction without requiring post-amplification analysis, reducing the possibility of contamination with the PCR product.
TB RQ kit provides a ready-to-use Real-Time PCR system for detection and quantitation of Mycobacterium tuberculosis complex DNA with Rotor-Gene (Qiagen) or StepOne (Applied Biosystems) machines.
Ready to Use
The TB Mix contains all necessary reagents for Real-Time PCR and is ready to use; no further preparation or mixing is required. Reactions are prepared simply by addition of the mix directly to the tubes followed by the sample DNA. Results can be viewed in FAM/Green channel.
PCR mix contains also specific DNA that is detected by a second probe in VIC/Yellow channel. Internal Control prevents false negative results by PCR inhibition or setup errors.
TB standards & mix are manufactured in batches, and each series is distributed after passing quality control tests for specificity, sensitivity, accuracy and Precision.
Easy & Quick Setup
The kit contains a template file for quick and easy setup of the Real-Time PCR instrument and no need to go through time consuming process of manual setting, also reducing setup errors.
Kit contains TB Mix, 5 quantitation standards, PCR grade water, manual and a CD with template file.
Kit is available as 24, 48 and 96 reactions of 25µl volume.
Technical support team is always available via phone or email. The team may also visit your lab for training or trouble shooting.
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