Quantitative Detection of PML-RARA Transcripts
PML-RARA is an abnormality resulted from t(15;17)(q22;q21) translocation. This translocation results in fusion of PML (promyelocytic) gene with RARA (retinoic acid receptor alpha) gene, and production of chimeric PML-RARA protein which is a transcription repressor and impairs the myeloid differentiation. While RARA breakpoints always occure in intron 2, PML breakpoints involves three different regions of intron 6 (55%), exon 6 (5%) and intron 3 (40%). The resulted isoforms of PML-RARA are respectively called bcr1/Long/L, bcr2/Variant/V and bcr3/Short/S.
PML-RARA accounts for more than 90% of APL (acute progranulocytic leukemia) cases, 10-15% of AML (acute myeloid leukemia) cases.
PML-RARA RQ kit provides a ready-to-use system for detection and quantitation of PML-RARA transcripts as well as calculation of PML-RARA percentage.
This kit is based on Real-Time PCR technology.
Advantages of PML-RARA RQ Kit
Ready to Use
The PML-RARA and ABL Mixes contain all necessary reagents for Real-Time PCR and are ready to use; no further reagent or mixing is required. Reactions are prepared simply by addition of the mix directly to the tubes followed by the sample cDNA. Results can be viewed in Green/FAM channel for PML-RARA and Yellow/VIC channel for ABL.
Kit also provides PCR mix and standards for assessment of ABL expression as the control gene. This would evaluate quality of the patient sample, RNA extraction and cDNA synthesis as well as preventing false negative results by PCR inhibition or setup errors.
Standards & mixes of PML-RARA RQ Kit are manufactured in batches, and each series is distributed only after passing Quality Control tests for Specificity, Sensitivity, Accuracy and Precision.
Easy & Quick Setup
Kit contains a template file for quick and easy setup of the Real-Time PCR instrument and no need to go through time taking manual setting, also reducing setup errors.
Kit contains PCR Mix and 5 quantitation standards for PML-RARA, PCR Mix and 4 quantitation standards for ABL, Primer and PCR grade water, manual and a CD with template file.
Kit is available as 24, 48 and 96 reactions of 25µl volume.
Technical support team is always available via phone or email. The team may also visit your lab for training or troubleshooting.
PML-RARA Quick Guide
PML-RARA RotorGene Templates