Novin Gene

Real-Time PCR Kits

MTHFR-677 RG Kit

Detection of MTHFR Mutation (C677T)

MTHFR stands for Methylenetetrahydrofolate reductase which is a key regulatory enzyme in folate and homocysteine metabolism. Since, the C677T and A1298C variants show reduced activity (30-65% and 15-30% respectively), these polymorphisms are among the the most important genetic determinants of plasma total homocysteine. Due to their influence on homocysteine metabolism, they have been linked to several complex disorders including arterial thrombosis, atherosclerosis, coronary heart disease, neural tube defects, several types of cancer, adverse drug interactions and osteoporosis though, findings are still controversial. Depending on the ethnic background up to 50% of population may carry the variant allele of MTHFR.

MTHFR-677 RG kit provides a ready-to-use Real-Time PCR system for detection of C677T variant with RotorGene machine.

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Description

Advantages of MTHFR677 RG Kit
 
 

Ready to Use

The MTHFR677 RG Mix contains all necessary reagents for
Real-Time PCR and is ready to use; no further reagent or mixing is required. Reactions are prepared simply by addition of the mix directly to the tubes followed by the sample DNA. Results can be viewed in Green/FAM channel melt curve.

 Internal Control

This kit requires no internal control as any sample should be positive for either or both of mutant and wild type allels. Therefore, the main reaction ensures quality of patient sample and DNA extraction and also prevents false negative results by PCR inhibition or setup errors.

Quality Control

mix & controls of MTHFR677 RG Kit are
manufactured in batches, and each series is distributed only after passing Quality Control tests for Specificity, Sensitivity and Accuracy.

 Easy & Quick Setup

The kit contains a template file for quick and easy setup of the Real-Time PCR instrument and no need to go through time taking manual setting, also reducing setup errors.

Kit Contents
Kit contains MTHFR Mix, MM Positive  control, WM Positive control, WW Negative control, PCR grade water, manual and a CD with template file.

Packaging
Kit is available as 24, 48 and 96 reactions of 25µl volume.

Technical Support
Technical support team is always available via phone or email. The team may also visit your lab for training or troubleshooting.

MTHFR-677 RG Manual
MTHFR-677 RG Quick Guide
MTHFR-677 RG RotorGene Templates

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