Novin Gene

Real-Time PCR Kits

mbcr 190 RQ Kit

Quantitative Detection of BCR-ABL p190 Translocation (Philadelphia Chromosome)

Philadelphia chromosome is an abnormality resulted from 9;22 translocation. Consequently ABL proto-oncogene on chromosome 9 is fused with BCR gene on chromosome 22. This fusion produces BCR-ABL protein, mostly 210kDa (b2a2 or b3a2) or 190kDa (e1a2), with constitutively active tyrosine kinase activity promoting cell proliferation and inhibition of apoptosis. The fusion gene transcript is detectable in about 95% of CML patients and some cases of ALL.  Also, serial monitoring of patients for identifying and measuring BCR-ABL transcripts provides more precise assessment of response to specific therapies and prediction of those in higher risk of disease progression.

MBCR 190 RQ provides a ready-to-use system for detection and quantitation of BCR-ABL transcripts (p190, e1a2 break point only) as well as calculation of BCR-ABL%.

This kit is based on Real-Time PCR technology.

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Description

Advantages of mbcr 190 RQ Kit

 

Ready to Use

mBCR and ABL Mixes contain all necessary
reagents for Real-Time PCR and are ready to use; no further reagent or mixing is required. Reactions are prepared simply by addition of the mix directly to the tubes followed by the sample cDNA. Results can be viewed in Green/FAM channel for BCR-ABL and Yellow/VIC channel for ABL.

 Control Gene

Kit also provides PCR mixes and standards for
assessment of ABL expression as the control gene. This would  evaluate quality of the patient sample, RNA extraction and cDNA synthesis as well as     preventing false negative results by PCR inhibition or setup errors.

 Quality Control

Standards & mixes of mBCR 190 RQ Kit  are manufactured in  batches, and each series is distributed only after passing Quality Control tests for Specificity, Sensitivity, Accuracy and Precision.

Easy & Quick Setup

The kit contains a template file for quick and easy setup of the Real-Time PCR instrument and no need to go through time consuming process of manual setting, also reducing setup errors.

Kit Contents

Kit contains PCR Mix and 5 quantitation standards for BCR-ABL, PCR Mix and 4 quantitation standards for .ABL, PCR grade water, manual and a CD with template file

Packaging

Kit is available as 24, 48 and 96 reactions of 25µl volume.

Technical Support

Technical support team is always available via phone or email. The team may also visit your lab for training or troubleshooting.

 

mbcr 190 RQ Manual
mbcr 190 RQ Quick Guide
mbcr 190 RQ RotorGene Templates
mbcr 190 RQ StepOne Templates

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