Novin Gene

Real-Time PCR Kits

HTLV-I RQ Kit

Quantitative Detection of HTLV-I provirus DNA Virus

Human T-cell Lymphotropic Virus I (HTLV-I) is the first human retrovirus discovered (1979). Two years later HTLV-II was also discovered. About 15 to 20 million people are infected with these viruses worldwide.  HTLV-I is endemic in North of Iran. Transmission is mostly through sexual contact, breastfeeding, Transfusion, Transplant and Intravenous drug use. Within the body, infection spreads slowly through virological synapses from cell to cell and takes 20-50 years to develop symptoms. Infection with HTLV-I may lead to leukemia/lymphoma or myelopathy while HTLV-II causes mild neurologic disorders or chronic pulmonary infection. Disease progress and pathogenesis depend partly on proviral load and monitoring that could be beneficial for patient management.

HTLV-I RQ kit provides a ready-to-use Real-Time PCR system for detection and quantitation of HTLV-I provirus and cellular titer (based on Albumin gene) with Rotor-Gene or StepOne machines. The Albumin gene also monitors extraction quality and prevents false negative results.

Description

Advantages of HTLV RQ Kit
 

Ready to Use

Both of the HTLV-I Mix and Albumin Mix
contain all necessary reagents for Real-Time PCR and are ready to use; no further reagent or mixing is required. Reactions are prepared simply by addition of the mix directly to the tubes followed by the sample DNA. Results for HTLV-I can be viewed in Green/FAM channel.

 Internal Control

Kit also contains a second set of PCR mix and standards for Albumin as internal
control and normalizer. Results can be viewed in Red/CY5 channel and provides sample
cellular load to normalize HTLV-I titer. Internal Control also prevents false negative results by PCR inhibition or setup errors.

 Quality Control

HTLV-I standards & mixes are manufactured in batches, and each series is distributed only after passing Quality Control tests for Specificity, Sensitivity, Accuracy and Precision.

 Easy & Quick Setup

The kit contains a template file for quick and easy setup of the Real-Time PCR machine and no need to go through time taking manual setting, also
reducing setup errors.

Kit Contents

Kit contains 2 series ofPCR  Mix and for (4) standards HTLV-I and Albumin, PCR grade water, manual and a CD with
template file.

Packaging

Kit is available as 24, 48 and 96 reactions of 25µl volume.

Technical Support

Technical support team is always available via phone or email. The team may also visit your lab for training or troubleshooting.

 

HTLV-I RQ Manual
HTLV-I RQ Quick Guide
HTLV-I RQ RotorGene Templates
HTLV-I RQ StepOne Templates

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