HTLV-I RQ Kit
Quantitative Detection of HTLV-I provirus DNA Virus
Human T-cell Lymphotropic Virus I (HTLV-I) is the first human retrovirus discovered (1979). Two years later HTLV-II was also discovered. About 15 to 20 million people are infected with these viruses worldwide. HTLV-I is endemic in North of Iran. Transmission is mostly through sexual contact, breastfeeding, Transfusion, Transplant and Intravenous drug use. Within the body, infection spreads slowly through virological synapses from cell to cell and takes 20-50 years to develop symptoms. Infection with HTLV-I may lead to leukemia/lymphoma or myelopathy while HTLV-II causes mild neurologic disorders or chronic pulmonary infection. Disease progress and pathogenesis depend partly on proviral load and monitoring that could be beneficial for patient management.
HTLV-I RQ kit provides a ready-to-use Real-Time PCR system for detection and quantitation of HTLV-I provirus and cellular titer (based on Albumin gene) with Rotor-Gene or StepOne machines. The Albumin gene also monitors extraction quality and prevents false negative results.
Ready to Use
Both of the HTLV-I Mix and Albumin Mix contain all necessary reagents for Real-Time PCR and are ready to use; no further reagent or mixing is required. Reactions are prepared simply by addition of the mix directly to the tubes followed by the sample DNA. Results for HTLV-I can be viewed in Green/FAM channel.
Kit also contains a second set of PCR mix and standards for Albumin as internal control and normalizer. Results can be viewed in Red/CY5 channel and provides sample cellular load to normalize HTLV-I titer. Internal Control also prevents false negative results by PCR inhibition or setup errors.
HTLV-I standards & mixes are manufactured in batches, and each series is distributed only after passing Quality Control tests for Specificity, Sensitivity, Accuracy and Precision.
Easy & Quick Setup
The kit contains a template file for quick and easy setup of the Real-Time PCR machine and no need to go through time taking manual setting, also reducing setup errors.
Kit contains 2 series ofPCR Mix and for (4) standards HTLV-I and Albumin, PCR grade water, manual and a CD with template file.
Kit is available as 24, 48 and 96 reactions of 25µl volume.
Technical support team is always available via phone or email. The team may also visit your lab for training or troubleshooting.