Novin Gene

Real-Time PCR Kits

HCV RQ Kit

Quantitative Detection of Hepatitis C Virus RNA

Hepatitis C virus (HCV) is an RNA virus and an important human pathogen. It is estimated that about 180 million individuals are infected worldwide. It can cause acute and chronic hepatitis followed by liver cirrhosis and hepatocellular carcinoma.

Currently infection can be diagnosed by detection of HCV RNA through RT-PCR in patient sample, weeks to months before antibody response. In addition, monitoring viral load by the same method has become a clinical gold standard for evaluation of therapy success as well as disease progress.

HCV RQ kit provides a ready-to-use One-Step Real-Time RT-PCR system for detection and quantitation of HCV RNA with Rotor-Gene or StepOne machines.

Description

Advantages of HCV RQ Kit

Ready to Use

The HCV Mix contains all necessary reagents for Real-Time One-Step RT-PCR and is ready to use; no further preparation or mixing is required. Reactions are prepared simply by addition of the mix directly to the tubes followed by the sample RNA. Results can be viewed in FAM/Green channel.

 Internal Control

RT-PCR mix contains also specific RNA that is detected by a second probe in VIC/Yellow channel. Internal Control prevents false negative results by RT-PCR inhibition or setup errors.

 Quality Control

HCV Standards & Mix are manufactured in batches, and each series is distributed only after passing Quality Control tests for Specificity, Sensitivity, Accuracy and Precision.

 Easy & Quick Setup

The kit contains a template file for quick and easy setup of the Real-Time PCR instrument and no need to go through time consuming process of manual setting, also reducing setup errors.

Kit Contents

Kit contains HCV Mix, 4 quantitation standards, PCR grade water, manual and a CD with template file.

Packaging

Kit is available as 24, 48 and 96 reactions of 25µl volume.

Technical Support

Technical support team is always available via phone or email. The team may also visit your lab for training or troubleshooting.

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