HCV Gen RG Kit
Hepatitis C Virus Genotyping
Hepatitis C virus (HCV) is the major cause of cirrhosis and hepatocellular carcinoma. So far 6 major genotypes and more than 80 subtypes have been identified.
Based on current clinical trials, pre-treatment viral load and HCV genotype are two best indicators for medication choice as well as patient response to therapy. Therefore, once patient tested positive for HCV, both viral load and genotype should be determined. Moreover since HCV genotypes have significant differences in their global distribution and prevalence, genotyping can be a useful tool for epidemiologic studies.
The gold standard method for HCV genotyping is sequencing and phylogenetic studies. Since this method is not feasible for routine practice in a clinical laboratory, other methods have been used for this purpose which most of them are PCR-based.
HCV Genotype RG kit provides a ready-to-use One-Step Real-Time RT-PCR system for genotyping of HCV types 1, 2 and 3.
Ready to Use
The HCV Gen Mix contains all necessary reagents for Real-Time One-Step RT-PCR and is ready to use; no further reagent or mixing is required. Reactions are prepared simply by addition of the mix directly to the tubes followed by the sample RNA. Results can be viewed in Green/FAM, Yellow/VIC and Red/CY5 channels.
HCV Gen mix & controls are manufactured in batches, and each series is distributed only after passing Quality Control tests for Specificity, Sensitivity and Accuracy.
Easy & Quick Setup
The kit contains a template file for quick and easy setup of the Real-Time PCR instrument and no need to go through time taking manual setting, also reducing setup errors.
Kit contains HCV Gen Mix, one Positive control for each of types, PCR grade water, manual and a CD with template file.
Kit is available as 24, 48 and 96 reactions of 25µl volume.
Technical support team is always available via phone or email. The team may also visit your lab for training or troubleshooting.
HCV Gen RG Manual
HCV Gen RG Quick Guide
HCV Gen RotorGene Templates