Detection and Quantitation of JC Virus

JC Virus is a member of polyomaviruses genus with a double stranded DNA genome. BK virus belongs to this genus as well. Early infection occurs in early childhood and without clinical symptoms. Then JC virus establishes a lifelong infection usually in kidney, central nervous system or lymphocytes. Usually about 80% of adult population are infected with this virus without noticing it. However, JCV may lead to significant consequences in patients with immune deficiency or very weak immune system such as people with HIV/AIDs, lymphoma, multiple sclerosis (MS) and transplant patients. In these cases, JCV reactivation may result in a serious brain infection called progressive multifocal leukoencephalopathy (PML). Therefore, it is crucial to monitor JCV titer in above mentioned target group.
JC RQ kit provides a ready-to-use Real-Time PCR assay for detection and quantitation of JC virus DNA.

Category: Infectious Diseases

Advantages of JC RQ Kit

Ready to Use

The JC Mix contains all necessary reagents for Real-Time PCR and is ready to use; no further preparation or mixing is required. Reactions are prepared simply by addition of the mix directly to the tubes followed by the sample DNA. Results can be viewed in Green/FAM and Yellow/VIC channels.

Internal Control

Kit also incorporates an Internal Control (IC) to identify possible extraction failure or PCR inhibition and preventing false negative results.

Quality Control

Standards & mix of JC RQ Kit are manufactured in batches, and each series is distributed only after passing Quality Control tests for Specificity, Sensitivity, Accuracy and Precision.

Easy & Quick Setup

Kit contains a template file for quick and easy setup of the Real-Time PCR instrument and no need to go through time taking manual setting, also reducing setup errors.

Kit Specifications

Analytical Sensitivity: 5 copies/µl for the cloned target

Linear Range: 100 million to 50 copies/µl for the cloned target

Reaction Type: Quantitative Real-Time PCR

Detection Method: TaqMan probe, Duplex (FAM, VIC)

Kit Contents

Kit contains PCR Mix, 4 quantitation standards and Internal Control for JC Mix and PCR grade water, manual and a CD with template file.

Packaging

Kit is available as 24, 48 and 96 reactions of 25µl.

Technical Support

Technical support team is always available via phone or email. The team may also visit your lab for training or troubleshooting.

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