Detection and Genotyping of Hepatitis C Virus

Hepatitis C Virus (HCV) is the major cause of cirrhosis and hepatocellular carcinoma. So far 6 major genotypes and more than 80 subtypes have been identified.
The gold standard method for HCV genotyping is sequencing and phylogenetic studies. Since this method is not feasible for routine practice in a clinical laboratory, other methods have been used for this purpose which most of them are PCR-based.
HCV Genotype RG kit provides a ready-to-use One-Step Real-Time RT-PCR assay for detection and differentiating of HCV types 1, 2 and 3.

Category: Infectious Diseases

Advantages of HCV Gen RG Kit

Ready to Use

The HCV Gen Mix contains all necessary reagents for Real-Time One-Step RT-PCR and is ready to use; no further reagent or mixing is required. Reactions are prepared simply by addition of the mix directly
to the tubes followed by the sample RNA. Results can be viewed in Green/FAM, Yellow/VIC and Red/Cy5 channels.

Quality Control

HCV Gen mix & controls are manufactured in batches, and each series is distributed only after passing Quality Control tests for Specificity, Sensitivity and Accuracy.

Easy & Quick Setup

The kit contains a template file for quick and easy setup of the Real-Time PCR instrument and no need to go through time taking manual setting, also reducing setup errors.

Kit Specifications

Analytical sensitivity: 25 copies/µl for the cloned target

Reaction type: Qualitative One-Step Real-Time RT-PCR

Detection Method: TaqMan probe, Multiplex (FAM, VIC, Cy5)

Kit Contents

Kit contains HCV Gen Mix, one Positive control for each types, PCR grade water, manual and a CD with template file.

Packaging

Kit is available as 24, 48 and 96 reactions of 25µl.

Technical Support

Technical support team is always available via phone or email. The team may also visit your lab for training or troubleshooting.

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